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Document Type |
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Thesis |
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Document Title |
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GENOTOXICITY OF WATERPIPE SMOKE IN BUCCAL CELLS السمية الجينية لدخان الشيشة في خلايا الفم |
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Subject |
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Faculty of Sciences > biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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In this study, we investigated invivo and invitro the genotoxic effect of waterpipe smoke and its condensate in buccal cells and peripheral blood leucocytes using the single cell gel electrophoresis technique. In addition, the polycyclic aromatic hydrocarbon (PAH) and the volatile organic compounds in the condensate was analysed using gas chromatography-mass spectrometry (GC/MS) technique. Twenty samples of buccal cells were collected from waterpipe smokers after completing their smoking session. Subjects were randomly selected from apparently healthy Saudi males 28-65 years of age, living in and around the metropolitan city of Jeddah. The smoker varied in the types of smoked materials (jurak and moassal) and the time of smoking session. They were categorized into three groups according to the number of smoking sessions per day. Buccal cells from 20 matched apparently healthy non-smokers were used as control. Results showed that waterpipe smoking of jurak and moassel caused DNA damage invivo in smoker buccal cells. The comet in these cells was 50-60%. Smoke condensate of jurak and moassel caused DNA damage >88% and comet invitro in peripheral blood leukocytes from healthy non-smokers. The pipe water condensate did not cause any DNA damage using comet test. Analysis of the jurak and moassel smokes for the presence of PAHs by GC-MS could not detect any of 15 PAHs analyzed. As for the volatile organic compounds, the following were detected: Propantriol, 2, 3-Dihydro- 3, 5- dihydroxy- 6- methyl- 4H- pyran- 4one, Vanillin, Nicotine, Dihydromethyljasmonat, Hydroxymethy furancarboxaldehyd, 1, 2- Propandiol and 3 Ethoxy- 4- hydroxybenzaldehyd. 3- Ethoxy- 4-hydroxybenzaldehyd is reported to be genotoxic in vitro in human lymphocytes. In conclusion, waterpipe smokes can cause DNA damage invivo in buccal cells of jurak and moassel smokers. Also the smoke condensate of both jurak and moassel caused invitro peripheral blood leukocytes DNA damage and determined by comet test. |
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Supervisor |
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Dr. Sufian Mohammed El- Assouli |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1435 AH
2014 AD |
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Co-Supervisor |
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Dr. Mohammad Ibrahim Mujallid |
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Added Date |
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Sunday, May 18, 2014 |
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Researchers
| هدباء جارالله آل عمرة | Al Amrah, Hadba JarAllah | Researcher | Master | |
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