Document Details

Document Type : Thesis 
Document Title :
POTENTIAL PROTECTIVE ROLE OF THYMOQUINONE ON EXPERIMENTALLY INDUCED ALZHEIMER RATS.
الدور الوقائي المحتمل للثيموكينون ضد مرض الزهايمر المستحدث تجريبيا في الفئران
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Aim: To evaluate the neuroprotective potential of thymoquinone (TQ) on the oxidative stress status of the brain in aluminum chloride (AlCl3)-induced Alzheimer’s disease (AD) in rats. Place and Duration of the Study: King Fahd Medical Research Center (KFMRC), King Abdulaziz University, Jeddah Saudi Arabia, April 2018- April 2019. Methodology: Thirty adult male Sprague Dawley albino rats were randomly divided into 3 groups. Group 1(Control): supplemented orally with 1ml/kg/day corn oil for 4 weeks. Group 2 (AD): supplemented orally with AlCl3 (17 mg/kg/day) for 4 weeks. Group 3 (TQ/AD): supplemented concomitantly with oral TQ (10 mg/kg/day) and AlCl3 (17 mg/kg/day) for 4 weeks. At the end of the experiment, spatial working memory was assessed using the Y-maze spontaneous alternation test. Then, serum levels of malondialdehyde (MDA) and glutathione peroxidase enzyme (GPx) were assessed. After that, the rats were sacrificed, and the brain was isolated, and a light microscopic examination of the hippocampus was performed. Finally, the brain homogenate content of amyloid beta (Aβ), tau protein and acetylcholine were biochemically determined. Results: The AD group showed a significant decrease in the spontaneous alternation performance (SAP%) in Y-maze. Also, in the AD group, serum MDA, Aβ and tau protein in the brain homogenate were significantly increased with a significant decrease of serum GPx and acetylcholine in the brain homogenate. Examination of hematoxylin and eosin (H&E)-stained sections of the hippocampus of the AD group revealed decreased thickness and disorganization of the pyramidal cell layer of CA1 and CA3 where many pyramidal cells lost their triangular shape and appeared shrunken. The molecular and polymorphic layers showed increased glial cells and congested blood capillaries. The dentate gyrus showed marked disorganization with some cell loss. Co-administration of TQ with AlCl3 in TQ/AD group, improved SAP% and significantly decreased serum MDA, Aβ, tau protein in the brain. It also increased serum GPx level and acetylcholine level in the brain. Also, TQ partially attenuated the histopathological changes in the hippocampus. Conclusion: TQ could mitigate the oxidative stress markers, neurodegenerative indices and histopathological alteration encountered in AD that all reflected on improving the cognitive behavior. This may implement TQ as an adjuvant medical strategy in ameliorating AD. 
Supervisor : Prof. Mohamed Bendary 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2020 AD 
Added Date : Monday, March 2, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد الأمين فيصل زاهرZaher, Mohammed Alameen FaisalResearcherDoctorate 

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