Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
الكشف والتشخيص الجزيئي للتلوث البكتيري للمياه والمحاليل المستخدمة و الناتجة عن وحدات الغسيل الكلوي في بعض مستشفيات مدينة جدة الكشف والتشخيص الجزيئي للتلوث البكتيري للمياه والمحاليل المستخدمة و الناتجة عن وحدات الغسيل الكلوي في بعض مستشفيات مدينة جدة |
|
Subject |
: |
Faculty of Sciences |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
The study aimed to investigate the microbiological quality of the treatment water, fluids that used in hemodialysis units and the waste water that produced from dialyzer effluent by isolation, biochemical and molecular identification of Gram positive and negative bacteria, determination the resistance and sensitive of tested bacterial strains for different kinds of used antibiotics. A total of 141 bacterial isolates were recovered from dialysis water samples. Twenty-one of them were isolated from treated water samples (RO) (point 1), 46 from water inside the hemodialysis machines (point 2) and 74 from waste water produced from dialysis machines (point 3). These samples were collected from two hemodialysis units of government hospitals (A&B), Jeddah during the period of the study. Preparation the serial dilutions for (poin3) samples and All isolated samples (point 1, 2& 3) were cultured on agar medium to count the viable bacteria, and then were compared with standards of bacterial count for (point1&2), after that, study the morphological characterization of cells and colonies and Gram stain according to Bergys. The cultures of isolates were inoculated on selective media such macConkey and blood Agar for identification by vitek 2 compact device. Ten bacterial strains were selected and genetically identified on species level by comparing the results of the gene sequence with the documented international gene bank. Gene sequencing obtained for gene 16S rRNA for each strain was recorded at the international Gene Bank (NCBI) and get accession numbers for each strain and phylogenetic analysis. Antibiotics susceptibilities patterns of identified bacteria were determined for fourteen antibiotics. The overall compliance of treated water from (points 1& 2) to (EDTA) association and National standard for viable bacterial counts of Kingdom Saudi Arabia (<100 c.f.u./ ml) was 62.5 % and 64.7 % respectively. The highest rate of total bacteria count was related to the waste water from (point 3) from both hemodialysis units (A&B). The overall distribution of isolated bacteria showed the highest dominance of Gram negative bacilli (51.57 %), following by Gram positive cocci (29 %) and Gram-positive bacilli (19.14 %). The bacterial isolates, could be arranged according to as follow: Bacillus cereus (17.7 %), Acinetobacter baumannii (17 %), Staphylococcus epidermidis (14.89 %), Staphylococcus warneri (14.18 %), Acinetobacter pitti (13.47 %), Escherishia coli (8.51 %), Stenotrophomonas maltophilia (7.80 %), Klebsiella pneumonia (4.96 %) and Arthrobacter arilaitensis (1.41 %), representing 99.52 % of all isolates from both haemodialysis units. All tested isolated strains showed resistance against to 3 or more antibiotics. These results indicated that the dialyzer effluents were the most important source of bacterial contamination with Bacillus sp., Stenotrophomonas sp., Escherishia coli, Acinetobacter sp. and after that, the water from dialysis machines as the source of Staphyloccus sp. and Acinetobacter sp. Therefore, a competent prevention programs is needed to eliminate bacterial contamination of dialysis water system and to prevent a health risk due to these opportunistic pathogens. |
|
Supervisor |
: |
Dr. Ola Ibrahim El-Hamshary |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1439 AH
2018 AD |
|
Co-Supervisor |
: |
Dr. Sherif Edris Ahmed |
|
Added Date |
: |
Tuesday, February 13, 2018 |
|
Researchers
| هديل شعلان العبدلي | Al-Abdaly, Hadeel Shalan | Researcher | Master | |
|
Back To Researches Page
|