Document Details

Document Type : Article In Journal 
Document Title :
Chemical and acidic denaturation of a homodimeric glutathione transferase mu class from Rhipicephalus (Boophilus) annulatus
التفكيك الكيميائى و الحامضى لإنزيم الجلوتاثيون ترانسفيريز ثنائى التجانس من العائلة ميو المنقى من قراد البقر ريبيسيفيلاس (بوؤفيلس) أنيولاتس.
 
Subject : Enzymology 
Document Language : English 
Abstract : The equilibrium unfolding of a mu class glutathione transferase from Rhipicephalus (Boophilus) annulatus (BaGSTM) has been performed using guanidinium chloride (GdmCl), urea and acid denaturation to investigate the unfolding intermediates. Protein transitions were monitored by intrinsic fluorescence and 8-anilino-1-naphthalenesulfonate (ANS) binding. The results indicate that unfolding of BaGSTM using GdmCl (0-4.0 M) is a multi-step process, i.e., at least two intermediates coexist in equilibrium. The first intermediate, a partially dissociated dimer, exists at low GdmCl concentration (less than 1.5 M). This intermediate undergoes dissociation into two monomers at GdmCl concentration between 1.5 and 2.0 M. The monomeric intermediate started to be completely unfolded at higher GdmCl concentrations (> 2.0 M). Unfolding using urea (0-8.0 M) and acid-induced structures as well as the ANS fluorescence in presence of different concentrations of GdmCl or urea confirmed that the unfolding is a multi-step process. The formation of a molten globule state (a monomeric intermediate) at pH less than 3.8 was suggested by the strong enhancement of fluorescence of ANS and protein concentration dependent. 
ISSN : 1687-157X 
Journal Name : J. of Genetic Eng. & Biotechnol 
Volume : 6 
Issue Number : 1 
Publishing Year : 1429 AH
2008 AD 
Article Type : Article 
Added Date : Saturday, January 15, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عمرو السيد الحكيمEl Hakim, Amr El SayedResearcherDoctorateaselhakim@hotmail.com
ياسر عزت شاهينShahein, Yasser EzzatResearcherDoctorate 
رجاء رضا حامدHamed, Ragaa RedaResearcherDoctorate 
عبد المنعم عبد الحميد عبد اللهAbd Allah, Abdul Monem Abdel HamidResearcherDoctorate 
يسري محمد حمديHamdey, Yosery mohamedResearcherDoctorate 

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