Document Details

Document Type : Article In Journal 
Document Title :
AFLATOXINS PERCENT RECOVERY OF CB AND IMMUNOAFFINITY COLUMN PROCEDURE IN BLACK AND GREEN TEA
نسبة معالجة الأفلاتوكسين و قابلية المناعة فى الشاء الأخضر و الأسود
 
Document Language : English 
Abstract : Although there is an Egyptian standard regulating the number of mold spores in tea, however, no specific permissible limits for aflatoxins level in tea is available. Moreover, None of the published methods can discriminate between the aflatoxins in the presence of the interfering materials of black and green tea. The current study aimed at evaluating two methods (CB and Affinity) for aflatoxin detection and determination in black and green tea. The assessment of the aflatoxin extraction procedures showed that the Affinity procedure in green tea was 3.7 times more efficient to recover aflatoxins than the CB procedure where the recoveries for total aflatoxins were 47.8 and 12.9% for Affinity and CB respectively. No significant differences were detected between the two methods in case of black tea. Different TLC developing systems were evaluated to overcome the problem of the masking materials. The developing system, chloroform: isopropanol (99:1) proved to be the best system to separate the aflatoxins from each other and from the area of the masking materials. Green tea samples collected from the Egyptian market contained higher concentration of total aflatoxins compared with black tea samples. Further investigations are needed to develop a specific procedure for the determination of aflatoxin in tea to maximize the percent recovery 
ISSN : 45566 
Journal Name : Journal of Egyptian society for Toxicity 
Volume : 22 
Issue Number : 1 
Publishing Year : 1422 AH
2000 AD 
Article Type : Article 
Added Date : Saturday, January 8, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد نصر الدين محمد جمعةGomaa, Mohamed Nasr Eldin mohamedInvestigatorDoctoratemngomaa@gmail.com

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