Document Details

Document Type : Article In Journal 
Document Title :
PURIFICATION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC
تنقية و تعيين سمية طحلب الأسولاتوريا بواسطة جهاز الفصل الكروماتوجرافى عالى الحساسية
 
Subject : toxicity 
Document Language : English 
Abstract : Aqueous extract of toxic Oscillatoria collected from the Solar Lake was purified using ultrafiltration technique and gel filtration column chromatography. Mouse bioassay was used to trace the toxicity during purification steps. Dose response curve of the purified material was compared to that of the crude extract. A reverse phase HPLC technique (isocratic elution with 2% acelonitrile aqueous solution adjusted lo pH 2.5 with phosphoric acid and UV detection at 254 nm) was suggested to detect the toxic compound in the crude cyanobacterial extracts of the Solar Lake. Toxic and non toxic fractions of the gel filtration were used to identify the responsible peak for toxicity by HPLC. 
ISSN : 2341-456 
Journal Name : J. Egypt. Soc. Toxicol 
Volume : 201 
Issue Number : 1 
Publishing Year : 1420 AH
1998 AD 
Article Type : Article 
Added Date : Saturday, January 8, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد نصر الدين محمد جمعةGomaa, Mohamed Nasr Eldin mohamedInvestigatorDoctoratemngomaa@gmail.com

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 28506.pdf pdf 

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