Document Details

Document Type : Article In Journal 
Document Title :
Aflatoxins percent recovery of CB and immunoaffinity column procedures in black and green tea.
نسبة استرجاع الأفلاتوكسين من طريقة CB و طريقة عمود الأمينوأفينتى فى كل من الشاى الأسود و الأخضر
 
Subject : mycotoxins 
Document Language : English 
Abstract : Although there is an Egyptian standard regulating the number of mold spores in gtea, however, no specific permissible limits for aflatoxins level in tea is available. Morever, Non of the published methods can discriminate between the aflatoxins in the presence of the interfering materials of black and green tea. The current study aimed at evaluating two methods (CB and Affinity) for aflatoxin detection and determination in black and green tea. The assessment of aflatoxin extraction procedure showed that the affinity procedures in green tea was 3.7 times more efficient to recover aflatoxin than the CB procedures where the recoveries for total aflatoxins were 47.8 and 12,9% for affinity and CB respectivel y. No significance differences were detected between the two methods in case of black tea. Different TLC developing systems were evaluated to overcome the problem of the masking materials. The developing system, chloroform : isopropanol (99:1) proved to be the best system to separate the aflatoxins from each other and from the area of the masking materials. Green tea samples collected from the Egyptuan market contained higher concentration of total aflatoxinc compared with black tea samples. Further investigation are needed to develop specific procedures for the determination of aflatoxin in tea to maximize the percent recovery. 
ISSN : 1110-127 
Journal Name : Journal of Egyptian society and toxicology 
Volume : 22 
Issue Number : 1 
Publishing Year : 1420 AH
2000 AD 
Article Type : Article 
Added Date : Tuesday, December 28, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
احمد محمد عايشAyesh, Ahmed MohamedInvestigatorDoctorateAesh58@yahoomai.com
محمد نصر الدين محمد جمعةGomaa, Mohamed Nasr Eldin MohamedInvestigatorDoctoratemngomaa@gmail.com
خيرية محمود نجيبNaguib, Khayria MahmoudInvestigatorDoctorateKhayria@yahoo.com

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