Applications of DNA Sequencing Workshop
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Introduction
The Center of Excellence in Genomic Medicine Research (CEGMR) at king Abdul Aziz University is pleased to announce the first international workshop on 'applications of DNA sequencing.
This workshop is organized by continuous education and outreach program at CEGMR as part of its mission in education, research and service to the community.
CEGMR is an established platform that provides cutting edge research and professional education. CEGMR strives to provide training courses, workshops, and conferences of great scientific importance to give an opportunity to scientists interested in this field.
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Objectives
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Understand how dideoxynucleotides can be used to obtain DNA sequence information in a chain termination assay. |
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Understand how sequence reading can be automated using fluorescent ddNTPs. |
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Be able to read sequence from a polyacrylamide gel chromatogram. |
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Understand that errors occur in sequence reads and how these errors can be detected by comparison to a consensus sequence. |
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Dates, Location & Duration
 The course will be held in the laboratories of CEGMR, King Fahd Medical Research Center based at King Abdul Aziz University. Lectures and practical sessions will be held daily from 8:00 a.m. to 4:00 p.m.
Documents and Certificates
 Participants who successfully complete the theoretical and practical parts will be awarded a certificate of completion.
Fees and Registration
 3000 SR will be charged. Fees will include break refreshments, lunch, all workshop materials and documentation. Registration is on the basis of first come first serve, therefore early registration is highly recommended. For more details, kindly refer to: Terms and Conditions
Program Schedule
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1st Day:
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Time
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Topic
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8.00 am - 9.00 am
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Registration
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9.00 am - 10.00 am
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Introduction to the workshop
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10.00 am - 10.20 am
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Coffee and tea break
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10.20 am - 11.20 am
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Overview on DNA sequencing
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11.20 am -12.20 pm
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Applications of sequencing
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12.20 pm - 1.00 pm
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Lunch time
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1.00 pm - 4.00 pm
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Lab Work: Isolation of DNA from whole blood
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2nd Day:
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Time
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Topic
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8.00 am - 10.00 am
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Lab work: PCR amplification of DNA using fragment specific PCR Primers
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10.00 am -10.20 am
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Coffee and tea break
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10.20 am - 11.20 am
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Gel electrophoresis
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11.20 am - 12.20 am
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Types of mutations and other applications of genetic analyzer
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12.20 pm - 1.00 pm
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Lunch time
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1.00 pm - 4.00 pm
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UV Trans illumination using gel documentation
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3rd Day:
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Time
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Topic
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8.00 am - 9.00 am
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Purification of products to be sequenced
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| 9.00 am – 10.00 am |
Lab work: First purification procedure |
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10.00 am – 10.20 am
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Coffee and tea break
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10.20 am - 12.20 pm
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Lab work: Gel electrophoresis for purified products
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12.20 pm - 1.00 pm
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Lunch time
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| 1.00 pm - 2.00 pm |
Cycle sequencing using big dye termination |
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2.00 pm - 4.00 pm
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Lab Work: Cycle sequencing
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4th Day:
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Time
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Topic
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8.00 am - 9.00 am
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Various methods 2nd purification of sequencing product
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| 9.00 am – 10.00 am |
Lab work: 2nd purification of labelled product |
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10.00 am – 10.20 am
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Coffee and tea break
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10.20 am - 12.20 pm
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Sample loading followed b automated sequencing
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12.20 pm - 1.00 pm
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Lunch time
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| 1.00 pm - 2.00 pm |
Data analysis of sequenced fragments, differentiating homozygotes from heterozygotes |
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2.00 pm - 4.00 pm
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Lab Work: Sequencing data analysis and storage
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