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Document Type |
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Thesis |
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Document Title |
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Cytotoxic Effect of Ochratoxin A in Albino Rat Kidney and the Possible Protective Role of Ajwa Dates التأثير السمي الخلوي للأوكراتوكسين (أ) في كلية الجرذ الأبيض والدور الوقائي المحتمل لتمر العجوة |
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Subject |
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Sciences Faculty for Girls |
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Document Language |
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Arabic |
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Abstract |
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Ochratoxin A (OTA) is a mycotoxin found in our food in small amounts. Kidney is the primary target for OTA. So, the aim of this study was to investigate the cytotoxic effect of a low repeated dose of OTA on the kidney, and the potential protective role of Ajwa date as a rich source in antioxidants against OTA cytotoxicity. Four groups of weaning rats were used: (1) control; (2) OTA-treated rats (289 µg/ kg/ day); (3) Ajwa date extract (1 g/ kg/ day) treated rats; and (4) Ajwa date extract + OTA treated rats. After treatment for 28 days (5 days/ week), a significant decrease in body weight, kidney weight and relative weight was detected in OTA treated rats. Serum creatinine and urea level was elevated. Also, significant histopathological changes were detected. The normal architecture of renal tissue was lost where renal corpuscles showed severe congestion, swollen glomeruli with significant decrease in the urinary space area led to undifferentiated renal corpuscles. The histological damage extended into kidney tubules exhibiting damaged brush border, degenerated and binucleated cells, karyomegalic or pyknotic nuclei. The ultrastrucutral changes included narrowing of the capillary lumen, swelling, proliferation and necrosis of many cells as well as expansion of the mesangial matrix, thickening of the glomerular basement membrane, and deterioration of the filtration barrier. In tubules, numerous apoptotic cells were observed. The mitochondria were severely swollen with disintegrated membranes. In Ajwa date extract group the renal tissue was normal as the control. Whereas, in Ajwa date extract + OTA group the severity of the lesions was significantly reduced. Our results indicated that OTA induced significant histopathological changes in the kidney and may has the potential to cause kidney failure, administration of Ajwa date showed a protective effect and ameliorated the lesions of OTA nephrotoxicity. |
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Supervisor |
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Dr. Suzan Abdu |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1431 AH
2010 AD |
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Co-Supervisor |
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Dr. Awatef Ali |
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Added Date |
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Monday, June 14, 2010 |
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Researchers
| شذا عيسى الأنصاري | Al-Ansari, Shatha Essa | Researcher | Master | |
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