Application of PCR in Diagnostic Molecular Genetics Workshop
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Introduction
The Center of Excellence in Genomic Medicine Research (CEGMR) at king Abdul Aziz University is pleased to announce the first international workshop on 'applications of PCR in diagnostic molecular genetics'.
The Center of Excellence in Genomic Medicine Research (CEGMR) at king Abdul Aziz University is pleased to announce the first international workshop on 'applications of PCR in diagnostic molecular genetics'.
CEGMR is an established platform that provides cutting edge research and professional education. CEGMR strives to provide training courses, workshops, and conferences of great scientific importance to give an opportunity to scientists interested in this field.
Objectives
Molecular genetics has many applications in diagnostics and its considered and integral part of many diagnostic laboratories worldwide. This workshop will provide an opportunity for participants to learn basic techniques related to DNA and RNA and their applications in molecular diagnostics. The participants will enjoy experimentation involving nucleotide extraction all the way through to PCRs and RT-PCRs. Most methods will revolve around gel electrophoresis and usage of gel documentation system.
Dates, Location & Duration
 The course will be held in the laboratories of the CEGMR, King Fahd Medical Research Center based at King Abdul Aziz University. Lectures and practical sessions will be held daily from 8:00 a.m. to 4:00 p.m.
Documents and Certificates
 Participants who successfully complete the theoretical and practical parts will be awarded a certificate of completion
Fees and Registration
 2000 SR will be charged. Fees will include break refreshments, lunch, all workshop materials and documentation. Registration is on the basis of first come first serve, therefore early registration is highly recommended.
For more details, kindly refer to: Terms and Conditions
Program Schedule
| 1st Day: |
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Time
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Topic
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8.00 am - 9.00 am
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Registration
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9.00 am - 10.00 am
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Introduction to the workshop
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10.00 am - 10.20 am
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Coffee and tea break
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10.20 am - 11.20 am
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An overview on PCR
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11.20 am -12.20 pm
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Components and Conditions for PCR optimization
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12.20 pm - 1.00 pm
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Lunch time
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| 1.00 pm - 2.00 pm |
Various methods of DNA extraction |
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2.00 pm - 4.00 pm
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Lab Work: Isolation of DNA from whole blood
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| 2nd Day: |
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Time
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Topic
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8.00 am - 9.00 am
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PCR amplification of DNA fragment
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9.00 am -10.00 am
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Lab work: PCR and thermal cycling
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10.00 am - 10.20 am
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Coffee and tea break
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10.20 am - 12.20 am
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Gel electrophoresis to check PCR products
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12.20 pm - 1.00 pm
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Lunch time
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1.00 pm - 4.00 pm
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Lab work: UV Trans illumination using gel documentation system followed by result interpretation
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| 3rd Day: |
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Time
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Topic
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8.00 am - 10.00 am
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Restriction Fragment Length Polymorphism (RFLP) and its applications.
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10.00 am – 10.20 am
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Coffee and tea break
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10.20 am - 12.20 pm
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Lab work: Restriction enzyme processing of amplified PCR products
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12.20 pm - 1.00 pm
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Lunch time
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| 1.00 pm - 2.00 pm |
PCR primer designing |
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2.00 pm - 4.00 pm
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Recombinant DNA technology and human genome project
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| 4th Day: |
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Time
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Topic
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8.00 am - 10.00 am
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Polyacrylamide gel electrophoresis, UV trans illumination using gel documentation system and interpretation of RFLP products.
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10.00 am - 10.20 am
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Coffee and tea break
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10.20 am - 12.20 am
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Genotyping and application in disease
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12.20 pm - 1.00 pm
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Lunch time
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1.00 pm - 4.00 pm
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Lab Work: Application of RT-PCR
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| 5th Day: |
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Time
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Topic
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8.00 am - 10.00 am
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RNA isolation from whole blood
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10.00 am - 10.20 am
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Coffee and break time
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10.20 am - 12.20 pm
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RT-PCR
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12.20 pm - 1.00 pm
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Lunch time
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1.00 pm - 4.00 pm
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Lab work: RT-PCR Gel electrophoresis, UV trans illumination using documentation system and gel interpretation
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