Application of FISH Workshop
|
| Home >> Workshops and Training Programs Advertisement >> Application of FISH Workshop |
 |
|
|
|
Introduction
The Center of Excellence in Genomic Medicine Research (CEGMR) at king Abdul Aziz University is pleased to announce the first international workshop on ' Application of Fluorescence In Situ Hybridization'.
This workshop is organized by continuous education and outreach program at CEGMR as part of its mission in education, research and service to the community.
CEGMR is an established platform that provides cutting edge research and professional education. CEGMR strives to provide training courses, workshops, and conferences of great scientific importance to give an opportunity to scientists interested in this field.
Objectives
 |
Participants will be exposed to a new avenue of genetic diagnosis called fluorescence in situ hybridization. |
 |
They will learn about the fundamentals of the technique, its importance and use in the modern medical practice |
|
They will be provided with an in-depth knowledge about its applications in prenatal, postnatal and cancer diagnosis. |
|
They will have an opportunity of “hand on” experience of the technique and the use of a fluorescence microscope. |
|
General Information
 Cytogenetics is one of the important specialties of medical genetics that is used widely in genetic diagnosis. It provides with valuable information on that the numerical and structural abnormalities of chromosomes to the referred patients suspected of having some genetic abnormality.
This includes tissue culture, cell harvesting, chromosome preparation, banding and analysis under a bright field microscope. However, many cryptic chromosomal rearrangements including micro-deletions, duplications or telomeric rearrangements are often invisible at the routine microscopic resolution. Fluorescence in situ hybridization (FISH), is a molecular cytogenetic method, where specific fluorescently labeled probes are hybridized to the chromosome spreads of the patient to detect these micro-deletions or duplications. It is also used in confirming cryptic translocations and for rapid detection of numerical aberrations. This technique is mostly used as an adjunct to routine karyotyping. It has wide range of applications in prenatal, postnatal and in cancer diagnosis, and today it has become one of the most essential component of genetic diagnosis.
Application of the technique and interpretation of FISH results demand vast experience and expertise in this specialized field. The workshop will be conducted by expert personnel in the field.
Dates, Location & Duration
 The course will be held in the laboratories of CEGMR, King Fahd Medical Research Center based at King Abdul Aziz University. Lectures and practical sessions will be held daily from 8:00 a.m. to 4:00 p.m.
Documents and Certificates
 Participants who successfully complete the theoretical and practical sections will be awarded a certificate of completion.
Fees and Registration
3000 SR will be charged. Fees will include break refreshments, lunch, all workshop materials and documentation. Registration is on the basis of first come first serve, therefore early registration is highly recommended.
For more details, kindly refer to: Terms and Conditions
Program Schedule
|
1st Day:
|
|
Time
|
Topic
|
|
8.00 am - 9.00 am
|
Registration
|
|
9.00 am - 10.00 am
|
Introduction to the workshop
|
|
10.00 am - 10.20 am
|
Coffee and tea break
|
|
10.20 am - 11.20 am
|
Overview of cytogenetics in medical diagnosis
|
|
11.20 am -12.20 pm
|
Principle of fluorescence in situ hybridization technique
|
|
12.20 pm - 1.00 pm
|
Lunch time
|
|
1.00 pm - 4.00 pm
|
Lab Work: Preparation of metaphase slide for FISH experiment
|
|
| |
|
2nd Day:
|
|
Time
|
Topic
|
|
8.00 am - 9.00 am
|
Clinical application of fluorescence in situ hybridization in medical practice
|
|
9.00 am -10.00 am
|
Types of probes used
|
|
10.00 am - 10.20 am
|
Coffee and tea break
|
|
10.20 am - 11.20 am
|
Probe labelling
|
|
11.20 am - 12.20 pm
|
Lab work: Hybridization of labeled DNA probe to cytogenetic prepared slide
|
|
12.20 pm - 1.00 pm
|
Lunch time
|
|
1.00 pm - 4.00 pm
|
Discussion on post hybridization processing and detection methods
|
|
| |
|
3rd Day:
|
|
Time
|
Topic
|
|
8.00 am - 10.00 am
|
Lab Work: Post hybridization and processing of directly labeled DNA probes
|
|
10.00 am – 10.20 am
|
Coffee and tea break
|
|
10.20 am - 12.20 pm
|
Overview of computerized image analysis
|
|
12.20 pm - 1.00 pm
|
Lunch time
|
|
1.00 pm - 4.00 pm
|
Lab Work: Application of fluorescent microscope in FISH signal analysis using image analysis software
|
|
| |
|
4th Day:
|
|
Time
|
Topic
|
|
8.00 am - 9.00 am
|
Prenatal FISH application
|
|
9.00 am - 10.00 am
|
Postnatal FISH application
|
|
10.00 am - 10.20 am
|
Coffee and tea break
|
|
10.20 am - 11.20 am
|
Oncology FISH application
|
|
11.20 am -12.20 pm
|
Microdeletions syndromes FISH application
|
|
12.20 pm - 1.00 pm
|
Lunch time
|
|
1.00 pm - 4.00 pm
|
Lab Work: FISH image analysis and interpretation using mage analysis software
|
|
| |
|
5th Day:
|
|
Time
|
Topic
|
|
8.00 am - 9.00 am
|
Her2/neu FISH application in breast cancer
|
|
9.00 am - 10.00 am
|
Nomenclature (ISCN) of FISH result
|
|
10.00 am - 10.20 am
|
Coffee and tea break
|
|
10.20 am - 12.20 pm
|
Lab Work: Analysis and interpretation of cancer cells using gene amplification probes
|
|
12.20 pm - 1.00 pm
|
Lunch time
|
|
1.00 pm - 4.00 pm
|
Result interpretation and reporting using different types of FISH probes
|
|
|
|
|